Part:BBa_K2996505:Design

Lure sequence and DBD binding site spaced by 15 base pairs

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 24
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 24
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 24
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This was to verify if lure sequence had any effect on the off-target detection.

Source

Point mutation PCR